Objective To observe the effect of adult cold storage on the longevity and reproductive capacity of female Nasonia vitripennis. Methods The female N. vitripennis emerged on different days were cold stored at 15 ℃ for different period. Their longevity, parasitic capacity and reproductive capacity were then measured. Results The longevity of female N. vitripennis was extended after cold storage at adult stage. However, as the cold storage time prolonged, the parasitic and reproductive capacities were compromised. At a cold storage time for less than 12 d, the impact on these capacities of female N. vitripennis was not remarkable. In contrast, after cold storage of over 12 d, the egg production and parasitic capacities declined linearly. After cold stored for 24 d, the parasitic ratio of Boettcherisca peregrina by N. vitripennis reduced to 35% from 85% before cold storage and the average emerging parasitoid progeny to 6 from 33 per fly pupa. However, the female adult rate and the body length of the progeny were not affected. Conclusion Adult cold storage extends the longevity of female N. vitripennis at the cost of reproductive capability.

Objective The study was conducted to determine the effects of frozen storage of Lucilia sericata pupae on the prolificacy of Nasonia vitripennis. Methods The length of growth stages, sizes of descendants, life span of the females, parasitic and  reproductive  ability  of  descendants  of  N. vitripennis  within  L. sericata  pupae  were  documented. Results Two  groups of N. vitripennis in L. sericata pupae, frozen at -20 ℃ and -70 ℃, respectively, demonstrated a growth period of approximately 13 days, the body length of female descendants about 1.8 mm and males about 1.2 mm, the life span of female descendants about 11 days, suggesting  no  significant  differences  when  compared  to  the  control  group (P＞0.05).  A  total  of  83% to 90% eclosed N. vitripennis could successfully parasitize natural L. sericata pupae, and 73% to 80% emerged, about 37 from each pupa, with the female to male ratio of about 90%, suggesting no significant differences when compared to the control group (P＞0.05). Conclusion Frozen storage of L. sericata pupae had no effects on the development and prolificacy of N. vitripennis descendants.

【Abstract】 Objective To study the effects of Boettcherisca peregrine pupae frozen storage on the reproduction of Nasonia vitripennis. Methods The developmental duration, body length, female longevity and reproduction of the parasitoid were tested after the pupae of   B.peregrine  were  frozen  for different times at -20 ℃ and -70 ℃.  Results The developmental durations of N.vitripennis progeny (F1) at B.peregrine pupae stored at -20 ℃ and -70 ℃ were 12-13 d, the body length of female and male progeny were about 2 mm and 1.4 mm, respectively, and the longevity of female progeny was about 11 days. The above indexes had no significant difference with the control. The parasitic ratio of F1 N.vitripennis to natural B.peregrine pupae was 90%-97%, and the emergence ratio of F1 progeny was about 80%-87%. There were about 40 progeny parasitoids emerged from one fly pupa, and the female ratio was about 90%, the examined indexes had no significant difference with the control. Conclusion Frozen storage of B.peregrine pupae had no effect on the development and quality of N.vitripennis progeny.

Objective The parasitic behavior of female Nasonia vitripennis was studied.Methods Dissecting the parasitized fly pupae,the eggs number of female N.vitripennis laid in different parts of the three species of fly pupae was counted.The effect of parasitic times on the fecundity of female N.vitripennis was studied.Results Feeding with 20% honey water,the longevity of female was prolonged significantly.The female N.vitripennis mainly laid eggs in the abdomen of its host flies,especially on the sternites.Mating or not had no significant effect on the parasitic ability of the female parasitoid,but the fecundity of the parasitoid was decreased with the increase of the parasitic times.Conclusion The parasitic ability of female N.vitripennis had correlation with the parasitic times.